Journal: Acta neuropathologica communications

Article Title: BDNF augmentation reverses cranial radiation therapy-induced cognitive decline and neurodegenerative consequences.

doi: 10.1186/s40478-024-01906-9

Figure Lengend Snippet: Fig. 2 Riluzole treatment restores hippocampal BDNF in the irradiated hippocampus. 10–12 weeks old WT male mice received cranial radiation therapy (RT) followed by riluzole (RZ) treatment (13 mg/kg) in drinking water for 6–7 weeks. An ELISA-based quantification of BDNF from the micro-dissected hippocampus showed RT-induced reductions in the RT + Vehicle group. Importantly, RZ treatment in the cranially irradiated mice showed significant restoration of BDNF levels. Data are presented as mean ± SEM (N = 6–10 mice per group). P values were derived from two-way ANOVA and Bonferroni’s multiple comparisons test

Article Snippet: BDNF levels were quantified using a commercially available ELISA kit (E-EL-M0203, Elabscience Biotechnology) and uncoated ELISA plates (Nunc MaxiSorp, Biolegend).

Techniques: Irradiation, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Frontiers in psychiatry

Article Title: Quetiapine Attenuates Schizophrenia-Like Behaviors and Demyelination in a MK-801-Induced Mouse Model of Schizophrenia.

doi: 10.3389/fpsyt.2020.00843

Figure Lengend Snippet: FIGURE 5 | Brain-derived neurotrophic factor (BDNF) protein level in the frontal cortex of mice in control (Con), quetiapine (Que), MK-801 (MK), and MK-801+quetiapine (MK+Que) groups. Quetiapine attenuated the decrease of BDNF level in the frontal cortex of MK-801 mice. n=5 in each group. Mice were treated with chronic quetiapine (10 mg/kg/day, intraperitoneally) for 28 days. From day 22 to 28, 1 h after the administration of quetiapine, the mice were administered MK-801 (2 mg/kg/day, subcutaneously). One day after behavioral testing, the mice were sacrificed for BDNF protein analysis by ELISA. Results are expressed as means ± S.E.M. *P < 0.05 vs. Con, #P < 0.05 vs. MK.

Article Snippet: The level of BDNF was quantified using a commercial Mouse BDNF ELISA kit (Fitzgerald Industries International, North Acton, MA) according to the manufacturer’s protocol.

Techniques: Derivative Assay, Control, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Neuroscience

Article Title: Adiponectin Mediates Running-Restored Hippocampal Neurogenesis in Streptozotocin-Induced Type 1 Diabetes in Mice

doi: 10.3389/fnins.2018.00679

Figure Lengend Snippet: Effects of streptozotocin on corticosterone and neurotrophic factors. Intraperitoneal administration of streptozotocin significantly increased (A) serum levels of corticosterone while significantly reduced (B) hippocampal levels of adiponectin and (C) hippocampal levels of brain-derived neurotrophic factor in wild-type non-runners. Student t -test ∗ P < 0.05, ∗∗ P < 0.005.

Article Snippet: The levels of corticosterone adiponectin and BDNF were determined using commercially available ELISA kits, including mouse CORT ELISA kits (Assay Designs, Enzo Life Sciences, Switzerland), mouse adiponectin ELISA kits (Adipogen Corporation, United States) and mouse BDNF ELISA kits (R & D system, United States) for detection in hippocampal tissues.

Techniques: Derivative Assay

Journal: Molecular pain

Article Title: Epigenetic regulation of spinal cord gene expression contributes to enhanced postoperative pain and analgesic tolerance subsequent to continuous opioid exposure.

doi: 10.1177/1744806916641950

Figure Lengend Snippet: Figure 3. Effects of morphine treatment, incision and the combination on spinal cord gene expression. Expression patterns of spinal Bdnf and Pdyn on (a) day 1 and (b) day 3 after incision. Expression of Bdnf was higher in morphine-treated plus incision animals one day after surgery. Increased expression changes for Pdyn were observed at both one and three days after incision for all groups, and greater enhancement of expression in the morphine plus incision group at both time points. Error bars: SEM, n ¼ 5/group; *p < 0.05, ***p < 0.001 for comparison with controls and #p < 0.05 for within groups comparisons. Data were analyzed by two-way analysis of variance (ANOVA) followed by Fisher post hoc test for multiple comparisons within each time point.

Article Snippet: BDNF concentrations were measured in duplicate by using mouse BDNF ELISA kit (Gen Way Biotech), and the dynorphin levels were assayed in duplicate by using Dynorphin EIA kit (Phoenix Pharmaceuticals) according to the manufacturer’s instructions.

Techniques: Gene Expression, Expressing, Comparison

Journal: Molecular pain

Article Title: Epigenetic regulation of spinal cord gene expression contributes to enhanced postoperative pain and analgesic tolerance subsequent to continuous opioid exposure.

doi: 10.1177/1744806916641950

Figure Lengend Snippet: Figure 4. Epigenetic effects of morphine treatment, incision and the combination on spinal Bdnf and Pdyn expression. Chromatin immunoprecipitation (ChIP) assays done on lumbar spinal tissue (a) day 1 and (b) day 3 after incision. ChIP assays showed that promoter regions of Bdnf and Pdyn were more strongly associated with aceH3K9 on day 1 after incision in morphine-treated group compared with morphine or incision alone groups. Error bars: SEM, n ¼ 5–6/group; *p < 0.05, ***p < 0.001 for comparison with controls and #p < 0.05, ##p < 0.01 for within groups comparisons. Data were analyzed by two-way analysis of variance (ANOVA) followed by Fisher post hoc test for multiple comparisons within each time point.

Article Snippet: BDNF concentrations were measured in duplicate by using mouse BDNF ELISA kit (Gen Way Biotech), and the dynorphin levels were assayed in duplicate by using Dynorphin EIA kit (Phoenix Pharmaceuticals) according to the manufacturer’s instructions.

Techniques: Expressing, Chromatin Immunoprecipitation, Comparison

Journal: Molecular pain

Article Title: Epigenetic regulation of spinal cord gene expression contributes to enhanced postoperative pain and analgesic tolerance subsequent to continuous opioid exposure.

doi: 10.1177/1744806916641950

Figure Lengend Snippet: Figure 5. Effects of chronic morphine treatment and incision on spinal cord BDNF and dynorphin protein levels. Enzyme immunoassay (ELISA) for BDNF and dynorphin protein levels in lumbar spinal cord segments were done to determine the time course of the elevated proteins after incision in mice previously exposed to morphine. (a) BDNF levels were elevated on days 1 and 3 but return to baseline values by day 6 after incision. (b) Dynorphin levels remain persistently elevated up to day 6 after incision. Error bars: SEM, n ¼ 6/group, *p < 0.05 and **p < 0.01 for comparison with baseline. Data were analyzed by one-way ANOVA followed by Fisher post hoc test multiple com- parisons tests.

Article Snippet: BDNF concentrations were measured in duplicate by using mouse BDNF ELISA kit (Gen Way Biotech), and the dynorphin levels were assayed in duplicate by using Dynorphin EIA kit (Phoenix Pharmaceuticals) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Molecular pain

Article Title: Epigenetic regulation of spinal cord gene expression contributes to enhanced postoperative pain and analgesic tolerance subsequent to continuous opioid exposure.

doi: 10.1177/1744806916641950

Figure Lengend Snippet: Figure 6. Effects of selective inhibition of spinal BDNF and dynorphin signaling. (a) Effects of selective antagonists of the tropomyosin- receptor-kinase (TrkB) and k-opioid receptors, ANA-12 and nor-BNI, respectively. Both drugs reduced hyperalgesia given on day 1 or 3 after surgery compared to vehicle treatment. (b) The administration of ANA-12 or nor-BNI attenuated the reduced morphine analgesic efficacy on day 1, but only nor-BNI was effective on day 3 after surgery in opioid-exposed group. Error bars: SEM, n ¼ 6/group, *p < 0.05, **p < 0.01 and ***p < 0.001 for comparison between treatments for each time point. Data for each time point were analyzed by one-way ANOVA followed by Sidak multiple comparisons tests.

Article Snippet: BDNF concentrations were measured in duplicate by using mouse BDNF ELISA kit (Gen Way Biotech), and the dynorphin levels were assayed in duplicate by using Dynorphin EIA kit (Phoenix Pharmaceuticals) according to the manufacturer’s instructions.

Techniques: Inhibition, Comparison

Journal: Brain and behavior

Article Title: The behavioral and physiological effects of high-fat diet and alcohol consumption: Sex differences in C57BL6/J mice.

doi: 10.1002/brb3.708

Figure Lengend Snippet: FIGURE 6 Whole-brain BDNF. When normalized to their respective sex controls, males overall exhibited small but significant decreases to BDNF compared to females. Black bars indicate water, and gray bars indicate 10% EtOH. The dashed line indicates 100% RC H2O expression. ‡ significant sex difference at p < .05

Article Snippet: A low target concentration (working dilution 1:2) of sample and sample diluent buffer was created and then used in a BDNF ELISA (Mouse BDNF PicoKine ELISA Kit, Boster Biological Technology Co., Pleasanton, CA, USA).

Techniques: Expressing